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Agent skills

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Agent skill

Amplicon Processing with DADA2

Infers exact amplicon sequence variants (ASVs) from demultiplexed 16S rRNA or ITS amplicon FASTQ with DADA2 - removing primers with cutadapt (--discard-untrimmed), learning a per-run error model (filterAndTrim -> learnErrors -> dada -> mergePairs), merging run-level tables with mergeSequenceTable...

ClaudeCodexOpenClawData & Analytics
GPTomics898
Agent skill

CLIP-seq Preprocessing

Preprocess CLIP-seq reads (eCLIP, iCLIP, iCLIP2, iCLIP3, irCLIP, PAR-CLIP, FLASH) with protocol-specific UMI extraction, adapter trimming, length filtering, and post-alignment PCR-duplicate collapse. Use when raw CLIP FASTQ must be turned into deduplicated, crosslink-preserving BAM input for peak...

ClaudeCodexOpenClawData & Analytics
GPTomics898
Agent skill

Adapter Trimming

Remove sequencing adapters from FASTQ files using Cutadapt and Trimmomatic. Supports single-end and paired-end reads, Illumina TruSeq, Nextera, and custom adapter sequences. Use when FastQC shows adapter contamination or before alignment of short reads.

ClaudeCodexOpenClawData & Analytics
GPTomics898
Agent skill

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Preprocess small RNA sequencing data with adapter trimming and size selection optimized for miRNA, piRNA, and other small RNAs. Use when preparing small RNA-seq reads for downstream quantification or discovery analysis.

ClaudeCodexOpenClawData & Analytics
GPTomics898
Agent skill

CLIP-seq Preprocessing

Preprocess CLIP-seq data including adapter trimming, UMI extraction, and PCR duplicate removal. Use when preparing raw CLIP, iCLIP, or eCLIP reads for peak calling.

ClaudeCodexOpenClawData & Analytics
David-Li04060
Apache-2.0View resource
Agent skill

Ribo-seq Preprocessing

Preprocess ribosome profiling data including adapter trimming, size selection, rRNA removal, and alignment. Use when preparing Ribo-seq reads for downstream analysis of translation.

ClaudeCodexOpenClawEducation & Research
GPTomics898
Agent skill

Small RNA Preprocessing

Preprocess small RNA sequencing data with adapter trimming and size selection optimized for miRNA, piRNA, and other small RNAs. Use when preparing small RNA-seq reads for downstream quantification or discovery analysis.

ClaudeCodexOpenClawEducation & Research
GPTomics898
Agent skill

CLIP-seq End-to-End Pipeline

End-to-end CLIP-seq pipeline from FASTQ to ENCODE-compliant binding sites, single-nucleotide crosslink maps, annotation, motifs, and (optionally) differential binding. Use when running the full Yeo lab eCLIP / iCLIP / iCLIP2 / iCLIP3 / irCLIP / PAR-CLIP analysis with SMInput control, protocol-spe...

ClaudeCodexOpenClawEducation & Research
GPTomics898
Agent skill

eDNA Metabarcoding Pipeline

End-to-end eDNA metabarcoding from raw amplicons to community ecology. Covers QC, primer removal (mandatory before DADA2 filterAndTrim), denoising with OBITools3 v3 (obi stats plural; DMS-based) or DADA2 ASVs (Callahan 2017), decontam combined method as screening-not-classifier (Davis 2018), tag-...

ClaudeCodexOpenClawEducation & Research
GPTomics898
Agent skill

Ribo-seq Pipeline

End-to-end Ribo-seq analysis from FASTQ to translation efficiency and ORF detection. Use when analyzing ribosome profiling data to study translation.

ClaudeCodexOpenClawEducation & Research
GPTomics898
Agent skill

Small RNA-seq Pipeline

End-to-end small RNA-seq analysis from FASTQ to differential miRNA expression. Use when analyzing miRNA, piRNA, or other small RNA sequencing data.

ClaudeCodexOpenClawEducation & Research
GPTomics898

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