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Agent skill

Long-Read Splicing Analysis

Analyzes alternative splicing from PacBio Iso-Seq (HiFi, Kinnex/MAS-Iso-seq) and Oxford Nanopore (direct cDNA, direct RNA, R10.4.1+) long-read RNA-seq with full-isoform resolution. Tools include FLAIR (correct/collapse/quantify/diffSplice for PacBio + ONT), IsoQuant (de-novo or annotation-guided ...

ClaudeCodexOpenClawData & Analytics
GPTomics1,232
Agent skill

Metagenome Assembly

Assembles microbial-community sequencing into metagenome-assembled genomes (MAGs) with metaFlye (ONT), metaSPAdes/MEGAHIT (Illumina), and hifiasm-meta/metaMDBG (PacBio HiFi), then recovers genomes via multi-binner consolidation (MetaBAT2, MaxBin2, CONCOCT, SemiBin2, VAMB -> DAS_Tool) and QCs them...

ClaudeCodexOpenClawData & Analytics
GPTomics1,232
Agent skill

bio-reads-qc-mapping

Ingest, QC, and map reads with reproducible outputs. Use for raw read processing and coverage stats.

OpenClawData & Analytics
fmschulz11
Agent skill

Metagenome Assembly

Metagenome assembly from long reads using metaFlye and metaSPAdes with binning strategies. Use when reconstructing genomes from microbial communities, recovering metagenome-assembled genomes (MAGs), or resolving strain-level variation in complex samples.

OpenClawData & Analytics
David-Li04060
Apache-2.0View resource
Agent skill

Iso-Seq Analysis

Analyze PacBio Iso-Seq data for full-length isoform discovery and quantification. Use when characterizing transcript diversity or identifying novel splice variants.

ClaudeCodexOpenClawData & Analytics
David-Li04060
Apache-2.0View resource
Agent skill

Medaka Polishing

Polishes Oxford Nanopore draft assemblies to higher consensus accuracy with medaka, a basecaller-model-specific neural consensus net, produces haploid variant calls (VCF) for microbial, mitochondrial, or viral samples, and generates amplicon/viral consensus sequences. Covers the model-matching fo...

ClaudeCodexOpenClawData & Analytics
GPTomics1,232
Agent skill

Full-Length Isoform Analysis

Discovers, classifies, filters, and quantifies full-length transcript isoforms from PacBio Iso-Seq/Kinnex (HiFi) and Oxford Nanopore (cDNA/direct-RNA) long reads, using the isoseq+pigeon pipeline, SQANTI3, and ONT tools (IsoQuant, FLAIR, Bambu, StringTie2). Covers why a novel isoform is an artifa...

ClaudeCodexOpenClawEducation & Research
GPTomics1,232
Agent skill

Long-Read Alignment with minimap2

Aligns Oxford Nanopore and PacBio long reads (and assemblies) to a reference with minimap2 using the error-rate-matched preset (map-ont, lr:hq, map-hifi, map-pb, splice/splice:hq, asm5/10/20, ava), producing a sorted/indexed BAM for variant, SV, methylation, or isoform analysis. Covers why the pr...

ClaudeCodexOpenClawEducation & Research
GPTomics1,232
Agent skill

m6Anet Direct-RNA m6A Detection

Detects m6A modifications from Oxford Nanopore direct-RNA-sequencing (ONT DRS) signal data using m6Anet (Hendra 2022 *Nat Methods* 19:1590; multiple-instance-learning neural network over DRACH 5-mer signal). Covers the required upstream pipeline (Dorado / Guppy basecalling -> minimap2 transcripto...

ClaudeCodexOpenClawEducation & Research
GPTomics1,232
Agent skill

Whole Genome Alignment

Build whole-genome alignments using Progressive Cactus (Armstrong 2020 reference-free clade-level WGA), Minigraph-Cactus (Hickey 2024 pangenome-aware), LASTZ chain/net (UCSC pipeline), MUMmer4 (Marçais 2018 pairwise), minimap2 -x asm5/10/20 (Li 2018 fast pairwise), AnchorWave (Song 2022 WGD-aware...

ClaudeCodexOpenClawEducation & Research
GPTomics1,232
Agent skill · Reference

bio-longread-medaka

Agent skill for Data Scientist, ML / AI Engineer, Researcher, published by Rudson-Oliveira. Project details and file access are provided by the publisher.

ClaudeCodexOpenClawData & Analytics
Rudson-Oliveira0
Not specifiedView resource
Agent skill · Reference

bio-longread-medaka

Agent skill for Researcher, ML / AI Engineer, Data Scientist, published by FridrichMethod. Project details and file access are provided by the publisher.

OpenClawEducation & Research
FridrichMethod16

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