Samtools BAM Processor Skill

---
name: samtools-bam-processor
description: BAM/SAM file manipulation skill for sorting, indexing, filtering, and extracting alignment data
allowed-tools:
  - Read
  - Write
  - Glob
  - Grep
  - Edit
  - WebFetch
  - WebSearch
  - Bash
metadata:
  version: "1.0"
  category: bioinformatics
  tags:
    - sequence-analysis
    - bam
    - sam
    - alignment
graph:
  domains: [domain:bioinformatics]
  specializations: [specialization:biomedical-informatics]
  skillAreas: [skill-area:python-data-pipelines, skill-area:data-analysis, skill-area:graph-algorithms]
  workflows: [workflow:experiment-design]
  roles: [role:research-engineer, role:lab-technician]
---

# Samtools BAM Processor Skill

## Purpose
Provide BAM/SAM file manipulation capabilities for sorting, indexing, filtering, and extracting alignment data.

## Capabilities
- BAM sorting and indexing
- Duplicate marking and removal
- Alignment statistics generation
- Region extraction and filtering
- Read group management
- Format conversion (SAM/BAM/CRAM)

## Usage Guidelines
- Sort and index BAM files for efficient access
- Mark or remove duplicates based on protocol
- Generate alignment statistics for quality assessment
- Extract regions of interest for targeted analysis
- Manage read groups for multi-sample data
- Use CRAM for storage efficiency

## Dependencies
- samtools
- Picard
- sambamba

## Process Integration
- Whole Genome Sequencing Pipeline (wgs-analysis-pipeline)
- Tumor Molecular Profiling (tumor-molecular-profiling)
- RNA-seq Differential Expression Analysis (rnaseq-differential-expression)